오가피로부터 분리된 조다당 분획물의 면역자극활성 및 Cisplatin과의 병용에 의한 항암 상승작용의 유도

• 영문명: Immunostimulation Activity of the Crude Polysaccharides Fractionated from Eleutherococcus senticosus, and its Application to Prevent of Tumors by Combination Therapy with Cisplatin
• 발행기관: 대한약학회
• 저자명: 하은숙(E. S. Ha) 황수현(S. H. Hwang) 유광원(K. W. Yu) 신광순(K. S. Shin) 조형민(H. M. Cho) 김창한(C. H. Kim) 박우문(W. M. Park) 윤택준(T. J. Yoon)
• 간행물 정보: 『약학회지』제47권 제3호 (2003년), 159~166쪽, 전체 8쪽
• 주제분류: 의약학 > 기타의약학
• 파일형태: PDF
• 발행일자: 2003.06.30

영문 초록

In order to study the clinical usefulness of crude polysaccharides fractionated from Eleutherococcus senticosus, EN-3, in eliminating tumors, we have investigated the effect of combination therapy on the murine tumor metastasis and growth models. In experimental metastasis of colon26-M3.1 cells, prophylactic intravenous (I.v.) administration of EN-3 (0.5, 5, and 50 μg/mouse) inhibited tumor metastasis compared with tumor control group in 33.6, 66.8, and 81.8% respectively, The administration of EN-3 (50 μg/mouse) also exhibited a 66.1% therapeutic effect on lung tumor metastasis. Although EN-3 induced no toxic effect on both tumor cell and normal splenocyte in the concentration below 100 μg/ml in in vitro, it induced significant proliferating activity on normal splenocyte in the concentration- dependent manner, In an analysis of NK-cell activity, I.v. adminisuation of EN-3 (4~100 μg/mouse) significantly augmented NK cytotoxicity to YAC-1 tumor cells. The combination treatments of cisplatin (10 μg) and EN-3 (5 μg) induced synergistic effect on the inhibition of tumor metastasis in experimental tumor metastasis model produced by colon26-M3.1 cells. In addition, the combination treatments also exhibited prolongation of lifespan in S-180 tumor bearing mouse for over the 60 days. Even though cisplatin (2.5 μg/ml) exhibited cytotoxicity to tumor cells and inhibited tumor growth over 95% in in vitro, combination treatment with EN-3 (20 μg/ml) was induced splenocyte proliferation and produced cytokines, such as TNF- α, IL-1 and IL-12, from the macmphages. These results suggested that EN-3 stimulate immune system non-specifically and apply to the biological response modifiers (BRM) in chemoimmunotherapy for tumor prevention.

키워드

Eleutherococcus senticosus BRM tumor metastasis immunostimulation activity chemo-immunotherapy.