오가피 조다당의 단백질 항원 (BSA and OVA)에 대한 면역증강효과

• 영문명: Effect of the Crude Polysaccharides Fraction from Eleutherococcus senticosus as a Immunoadjuvant to Soluble Antigens (BSA and OVA)
• 발행기관: 대한약학회
• 저자명: 황수현(S. H. Hwang) 하은숙(E. S. Ha) 유광원(K. W. Yu) 신광순(K. S. Shin) 이상훈(S. H. Lee) 이재권(J. K. Lee) 이경호(K. H. Lee) 윤택준(T. J. Yoon) 박우문(W. M. Park)
• 간행물 정보: 『약학회지』제47권 제3호 (2003년), 167~175쪽, 전체 9쪽
• 주제분류: 의약학 > 기타의약학
• 파일형태: PDF
• 발행일자: 2003.06.30

영문 초록

Eleutherococcus senticosus is a typical oriental folk medicinal herb. It has been used clinically as a anti-rheumatic disease, anti-stress, ischemic heart disease and gastric ulcer, In the present study, we examined the adiuvant activity of the crude polysactharides fraction from Eleutherococcus senticosus, EN-3, on the induction of humoral and cellular immune responses against bovine serum albumin (BSA) or ovalbumin (OVA). The thioglycollate-induced macrophages and silica-induced dendritic-like cells cultured with BSA and EN-3 synergistically increased the production of TNF-α and IL-12. When mice were subcutaneously immunized with BSA + EN- 3, the antibody titer against BSA was showed significantly higher than those immunized with BSA alone. In addition, when mice were immunized with 0VA + FIA + EN- 3, the antibody titer was showed similar patterns with the FCA. The assay for determining subisotype of antibody revealed that EN-3 augmented OVA-specific antibody titer of IgG1 and IgG2b. The culture supernatant obtained from splenocytes of mice treated with OVA + FIA + EN-3 also showed a higher level of both 0VA-specific Th1- type (IL-2, IFN-γ and GM-CSF) and Th2-type cytokine (IL-4, IL-6 and IL-10). In vitro analysis of T cell proliferation to OVA on 8 weeks, the splenocytes of mice treated with OVA + EN-3 showed a significantly higher proliferating activity than those treated with OVA alone. These results suggest that EN-3 may possess adjuvant activities to potentially to enhance humoral as well as cellular immune response.

키워드

Eleutherococcus senticosus adjuvant antibody T cell cytokines.