천연물로부터 티로시나제 활성 저해제의 검색

• 영문명: Screening of Inhibitors against Tyrosinase Activity from Natural Products
• 발행기관: 대한약학회
• 저자명: 최상숙(Sang Sook Choi) 노향순(Hyang Soon Noh) 조성희(Sung Hee Cho) 공광훈(Kwang Hoon Kong)
• 간행물 정보: 『약학회지』제45권 제5호 (2001년), 522~528쪽, 전체 7쪽
• 주제분류: 의약학 > 기타의약학
• 파일형태: PDF
• 발행일자: 2001.10.31

영문 초록

Tyrosinase plays an important role in the process of melanin biosynthesis, and it is a biochemical target enzyme for skin-whitening agents and the remedy for disturbances in pigmentation. We have screened 25 natural plant extracts for inhibitory effects against DOPA oxidate activity of tyrosinase. For the inhibitory effect against DOFA oxidase activity of tyrosinase, the recombinant human tyrosinase and the purified mushroom tyrosinase were used. Each of the dried plants extracted with methanol, and then the extracts were subjected to sequential fractionations with methylene chloride, ethyl acetate, n-butanol and waters respectively, The methylene chloride fractions of Angelica tenuissima, Nardcstachys chinensis, Bombyx mori and Saposhnikovia divaricata, and the n-butanol fraction of Bombyx mori notably inhibited the human tyrosinase activity as well as mushroom tyrosinase activity (more than 90% inhibition). This study suggests that the above extracts have a potential as whitening agents as single ingredients or in combination with other of the extracts.

키워드

Tyrosinase natural products.