반묘 BuOH층의 U937세포주에 대한 apoptosis유도 효과

• 영문명: Effect of Butanol Fraction of Mylabris phalerata on Induction of Apoptosis in U937 cells
• 발행기관: 대한약학회
• 저자명: 허정은(Jeong Eun Huh) 윤택준(Taek Joon Yoon) 이종수(Jong Soo Lee) 정진홍(Jin Hong Jung) 김성훈(Sung Hoon Kim)
• 간행물 정보: 『약학회지』제45권 제5호 (2001년), 484~490쪽, 전체 7쪽
• 주제분류: 의약학 > 기타의약학
• 파일형태: PDF
• 발행일자: 2001.10.31

영문 초록

Mylabris phalerata (MP) is an insect that has been used for the treatment of cancer in oriental medicine. To evaluate the anticancer activity of Mylabris phalerata, We measured the cytotoxicity of Mylabris phalerata solvent fractions such as MC, EA, BuOH and residual layers on U937, human monocytic leukemia cells. Of those fractions BuOH layer of Mylabris phalerata was the moat effective with IC50 of 140 μg/ml. It effectively caused DNA fragmentation from the concentration of 50μg/ml, showed apoptotic nucleus by tenet assay and expressed apototic portion stained by Annexin-V It also induced the activation of caspase-3 and cleavage of the substrate poly (ADP-ribose) polymerase (PARP). These results suggest BuOH layer of Mylabris phalerata exerts anticancer activity by induction of apoptosis via activation of caspase-3 protease.

키워드

Mylabris phalerata U937 DNA fragmentation caspase-3 PARP.