흡광도측정법에 이한 황백과 제제 중 프로토베르베린 알칼로이드의 정량

• 영문명: Determination of Protoberberine Alkaloids in Phellodendri Cortex and Preparation by Spectrophotometric Method
• 발행기관: 대한약학회
• 저자명: 엄동옥(Dong-Ok Eom) 정윤철(Yun-Cheol Jeon)
• 간행물 정보: 『약학회지』제45권 제1호 (2001년), 34~38쪽, 전체 5쪽
• 주제분류: 의약학 > 기타의약학
• 파일형태: PDF
• 발행일자: 2001.02.28

영문 초록

The Phellodendri Cortex of phellodendron amurense (Rutaceae) is known to contain a numher of isoquinoline alkaloid, and berberine, palmatine, jateorrhizine, phellodendrine and magnoflorine are the major constituents of protoberberine lkaloids. For the determination of protoberberine alkaloids from Phellodendri Cortex and berberin chloride from the preparation, the new spectrophotometric method was developed with a simple and selective sample clean-up using thiocyanatocobaltate[II] complex ion. Samplex were extracted with 0.1 nM hydrochloric acid, potassium bipthalate reagent, thiocyanatocobaltate reagent and 1.2-dichloroethane for 60 min. The absorbance of protoberberine alkaloid complexes in 1.2-dichloroethane solution was meaured at 625nm. Calibration curve for berberine was linear over the concentration range of 0.05~0.30mg/ml 1.2-dichloroethane. The method proved to be rapid, simple and reliable for the determination of protoberberine alkaloids from Phellodendri Cortex and berberine chloride from the preparation.

키워드

Phellodendri Cortex and preparation protoberberine alkaloid berberine chloride spectrophotometric method