눈꽃동충하초 (Paecilmyces japonica DGUM 32001) 균사배양물의 항암 효과에 관한 유세포분석학적연구

• 영문명: Flow Cytometrical Investigation on Antitumor Activity of Mycelial Culture of Insect-born Fungus Paecilomyces japonica DGUM 32001
• 발행기관: 대한약학회
• 저자명: 이지선(Ji-Seon Lee) 이임선(Im Seon Lee) 정경수(Kyeong-Soo Chung) 김용해(Yong Hae Kim) 한영환(Young Hwan Han) 이만형(Man Hyong Lee)
• 간행물 정보: 『약학회지』제45권 제1호 (2001년), 64~70쪽, 전체 7쪽
• 주제분류: 의약학 > 기타의약학
• 파일형태: PDF
• 발행일자: 2001.02.28

영문 초록

Protein-polysaccharide fractions, PJ-3 and PJ-4, were prepared from mucelial culture filtrate of an insect fungus, Paecilomyces Japonica DGUM 32--1, and subjected to a flow cytomerical analysis for their in vivo antitumor and immunomodulating activity in ICR mice. When I.p. injected once daily for seven days at 100mg/kg, PJ-4 exerted a strong antitumor activity, showing the growth inhibition ratio of 85.1% against I.p.implanted sarcoma 180 cells, while PJ-3 showed only a weak activity. Moreover, PJ-4 significantly increased the expression level of CD25 (IL-2R alpha-chain) as well as forward scatter (FSC) values of splenic CD8+ T cells. It is also noteworthy that PJ-4 strongly induced the peritoneal exudate cells in the same experiment. In an in virto study, PJ-4 slightly inhibited the growth of sarcoma 180 sells at toe consentration of 50 concentration of 50ug/ml or higher. These results strongly suggest that PJ-4 might exert its antitumor activity through immunostimulation as well as direct inhibitory activity on the tumor cells.

키워드

Basidiomycetes Paecilomyces japonica protein-polysaccharide antitumor sarcoma 180 immunomodulation flow cytometry