항 단실 항체의 카르보닐탄소 유래 시그날의 귀속

• 영문명: Assignment of the Carbonyl Carbon Resonances in Anti-Dansyl Antibodies
• 발행기관: 대한약학회
• 저자명: 김하형(Ha Hyung Kim) 이광표(Kwang Pyo Lee) Koichi Kato Yoji Arata
• 간행물 정보: 『약학회지』제39권 제5호 (1995년), 516~520쪽, 전체 5쪽
• 주제분류: 의약학 > 기타의약학
• 파일형태: PDF
• 발행일자: 1995.10.30

영문 초록

The anti-dansyl antibodies were specifically labeled with stable isotope by growing hybridoma cells in serum-free medium. Assignments of the observed carbonyl carbon resonances have been determined by using 13C-15N double labeling method in order to assign the Leu resonances. However, when the identical dipeptide appears more than twice in the polypeptide sequences, we applied the proteolytic fragments in the fragment-specific method. Carboxypeptidase B-treated antibody has also been used to assign the Lys-447 in C terminal amino acid. These unambiguously assigned carbonyl carbon resonances in antibodies are thought to be useful in elucidating not only the structure of antibodies but also the structure-function relationship in the antibody by 13C neuclear magnetic resonance spectroscopy.