Purification and Characterization of Mongolian Mare Lactoferrin

• 발행기관: 한국축산식품학회
• 저자명: Kee Sung Kim Ji Sun Kim Mi Soon Shin Hae Won Noh Sang Dong Lim Duvjir Suvd J. Alimaa
• 간행물 정보: 『Food Science of Animal Resources』Korean Journal for Food Science of Animal Resources 제29권 제2호, 164~167쪽, 전체 4쪽
• 주제분류: 농수해양 > 식품과학
• 파일형태: PDF
• 발행일자: 2009.03.01

영문 초록

The lactoferrin from mongolian mare colostrum has been purified by gel filtration (Sephadex G-100), affinity chromatography (Toyopearl-AF-Heparin-650M) in two steps. Mare lactoferrin-containing fractions were identified in the first peak among 3 peaks on Sephadex G-100 as first step, and purified lactoferrin was eluted with a step gradient of 0.5 M NaCl as a 3 step (gradient 0.1, 0.3, 0.5M). Eluted fractions were analyzed by 12% SDS-PAGE, and showed a single protein. Its molecular weight was estimated to be 82 kDa. N-terminal amino acid sequence was determined as APRKSVRWCTISPAEX-AKXA.

키워드

Lactoferrin mare milk molecular weight N-terminal sequence