Purification and Characterization of a Protease from Korean Pear (Pyrus serotina L.) as Meat Tenderizer

• 발행기관: 한국축산식품학회
• 저자명: Hao Li Guan P. K. Mandal Hee Kyong Lim Oyungerel Baatartsogt Chi Ho Lee Gwang Joo Jeon Il Shin Choe
• 간행물 정보: 『Food Science of Animal Resources』Korean Journal for Food Science of Animal Resources 제29권 제2호, 157~163쪽, 전체 7쪽
• 주제분류: 농수해양 > 식품과학
• 파일형태: PDF
• 발행일자: 2009.03.01

영문 초록

This study was conducted for the isolation, purification, and characterization of a protease from Korean pear, to see its proteolytic activity on chicken actomyosin and to find the optimum pH and temperature of activity on chicken actomyosin. The protease was isolated from crude extract of Korean pear by ammonium sulfate precipitation. Further purification was done by DEAE-Sepharose ion-exchange chromatography, Mono-Q and Mini-Q column chromatography. The purified enzyme gave a single protein band on SDS polyacrylamide gel electrophoresis and the molecular weight was found to be 38 kDa. The specific activity of purified enzyme was 34,907 unit/mg with 25 fold purification and the yield was 2%. The purified enzyme incubated with chicken actomyosin showed high activity. The optimum pH and temperature for enzyme activity on chicken actomyosin were 6.5 and 70℃, respectively. A protease was purified from Korean pear for the first time and characterized. It was found to be promising for meat tenderization.

키워드

Korean pear protease characterization proteolytic activity chicken actomyosin tenderizer