흰쥐의 Nucleus Raphe Magnus로부터 분리된 신경세포의 전기생리학적 성질

• 영문명: Electrophysiological Properties of the Neurons Dissociated from the Nucleus Raphe Magnus in Postnatal Rats
• 발행기관: 대한생리학회-대한약리학회
• 저자명: 남상채(Sang Chae Nam) 임원일(Won il Lim) 조사선(Sa Sun Cho) 김전(Jun Kim)
• 간행물 정보: 『The Korean Journal of Physiology & Pharmacology』제1권 제3호, 233~240쪽, 전체 8쪽
• 주제분류: 의약학 > 의학일반
• 파일형태: PDF
• 발행일자: 1997.01.01

영문 초록

Neurons in the nucleus raphe magnus are involved in descending modulation of nociceptive transmission. In this study, we attempted to investigate electrophysiological properties of the NRM neurons dissociated from the postnatal rat medulla. The NRM neurons in the coronal slices of and the dissociated neurons from the postnatal rat medullae were immunohistochemically identified using antibody against serotonin. Relatively small number of neurons were positively stained in both preparations. The positively stained neurons displayed large cell body with double or multiple neurites. Using whole-cell patch clamp configuration ionic currents were recorded from the dissociated NRM-like neurons selected by criteria such as size and shape of cell body and cell population. Two types, high- and low-threshold, of voltage-dependent calcium currents were recorded from the dissociated NRM-like neurons. Some neurons displayed both types of calcium currents, whereas others displayed only high- threshold calcium current. Voltage-dependent potassium currents were also recorded from the dissociated NRM neurons. Some neurons displayed both transient outward and delayed rectifier currents but others showed only delayed rectifier current. These results suggest that there are at least two types of calcium currents and two types of potassium currents in the dissociated NRM neurons.

키워드

Nucleus raphe magnus Medulla Serotonin antibody Calcium current Potassium current