일측 전정기관 손상 흰쥐에서 전정안구반사와 내측전정신경핵의 c-Fos 단백질발현에 대한 전정기관의 전기자극 효과

• 영문명: Effect of Electrical Stimulation of the Vestibular System on Vestibuloocular Reflex and c-Fos Expression in the Medial Vestibular Nuclei of Unilateral Labyrinthectomized Rats
• 발행기관: 대한생리학회-대한약리학회
• 저자명: 박병림(Byung Rim Park) 황호룡(Ho Ryong Hwang) 이문영(Moon Yong Lee) 김민선(Min Sun Kim)
• 간행물 정보: 『The Korean Journal of Physiology & Pharmacology』제1권 제3호, 275~284쪽, 전체 10쪽
• 주제분류: 의약학 > 의학일반
• 파일형태: PDF
• 발행일자: 1997.01.01

영문 초록

Unilateral labyrinthectomy (ULX) causes autonomic symptoms, ocular and postural asymmetries, which disappear over time in the process of equilibrium recovery known as vestibular compensation. In the present study, in order to elucidate mechanisms responsible for the effects of electrical stimulation on vestibular compensation and investigate the relationship between vestibular compensation and c-Fos expression in the medial vestibular nuclei following ULX, we measured spontaneous nystagmus, eye movement induced by sinusoidal rotation and c-Fos expression up to 72 hs after ULX in Sprague-Dawley rats. Experimental animals were divided into two groups: ULX group with ULX only, and electrical stimulation (ES) group with electrical stimulation of ⁣2∼⁣5 V, 1.0 ms, 100 Hz to the lesioned vestibular system for 4 hs/day. Spontaneous nystagmus following ULX disappeared by 72 hs in ULX group and 36 hs in ES group. In eye movement induced by sinusoidal rotation, normal pattern of eye movement by rotation toward the lesioned side was recovered 24 hs after ULX at rotation of 0.1 Hz and 6 hs after at 0.2 Hz, 0.5 Hz in ULX group. In ES group, the eye movement recovered after 12 hs at 0.1 Hz, 6 hs at 0.2 Hz, and 4 hs at 0.5 Hz. Directional preponderance which represents the symmetry of bilateral vestibular functions showed significantly early recovery in ES group compared with that of ULX group. Expression of c-Fos immunoreactive cells in the bilateral medial vestibular nuclei was severely asymmetrical till 36 hs in ULX group, and then it became a symmetry and disappeared after 72 hs. However, ES group showed the symmetry of c-Fos expression after 6 hs, which was significantly early recovery in ES group. All these findings suggest that electrical stimulation ameliorates recovery of vestibuloocular reflex following ULX by the restoration of the balance of the resting activity between bilateral medial vestibular nuclei. In addition, c-Fos expression in the medial vestibular nuclei could be used as a marker of vestibular compensation since c-Fos expression is closely related to the course of recovery following ULX.

키워드

Vestibular compensation Electrical stimulation Vestibuloocular reflex Spontaneous nystagmus c-Fos Medial vestibular nuclei Rat