신경병증성 통증을 유발한 흰쥐에서 신경손상부위에 따른 배근신경절 및 척수의 신경전달물질의 변동

• 영문명: The Changes of Immunoreactivity for CGRP and SP in the Spinal Cord and DRG According to the Distance between the DRG and Injury Site of a Peripheral Neuropathic Rat
• 발행기관: 대한생리학회-대한약리학회
• 저자명: 김희진(Hee Jin Kim) 김우경(Woo Kyung Kim) 백광세(Kwang Se Paik) 강복순(Bok Soon Kang)
• 간행물 정보: 『The Korean Journal of Physiology & Pharmacology』제1권 제3호, 251~262쪽, 전체 12쪽
• 주제분류: 의약학 > 의학일반
• 파일형태: PDF
• 발행일자: 1997.01.01

영문 초록

Peripheral nerve injury sometimes leads to neuropathic pain and depletion of calcitonin gene related-peptide (CGRP) and substance P (SP) in the spinal cord. However, the pathophysiological mechanisms for depletion of CGRP and SP following the neuropathic injury are still unknown. This study was performed to see whether the distribution of immunoreactivity for CGRP and SP in the superficial dorsal horn and dorsal root ganglia(DRG) was related to the distance between the DRG and injury site. To this aim, we compared two groups of rats; one group was subjected to unilateral inferior and superior caudal trunk transections at the level between the S3 and S4 spinal nerves (S34 group) and the other group at the levels between the S1 and S2, between S2 and S3 and between S3 and S4 spinal nerve (S123 group). The transections in both groups equally eliminated the inputs from the tail to the S1-3 DRG, but the distance from the S1/S2 DRG to the injury site was different between the two groups. Immunostaining with SP and CGRP antibody was done in the S1-S3 spinal cord and DRG of the two groups 1 and 12 weeks after the injury. The results obtained are as follows:

1. The immunoreactivity for CGRP and SP in the ipsilateral superficial dorsal horn and DRG decreased 1 and 12 weeks after neuropathic nerve injury.

2. The immunoreactive area of SP and CGRP in the S1 dorsal horn was smaller in the S123 group than in the S34 group, whereas that in the S3 dorsal horn was not significantly different between the two groups. The number of SP- immunoreactive DRG cells decreased on the neuropathic side as compared to the sham group s in all DRGs of experimental groups except the S1 DRG of the S34 group.

These results suggest that the amounts of SP and CGRP in the dorsal horn and DRG following neuropathic injury inversely decrease according to the distance between the DRG and injury site.

키워드

Peripheral nerve injury Allodynia Substance P Calcitonin gene- related peptide