아세틸콜린 수용체 항체(Acetylcholine receptor autoantibody) 검사의 의의

• 영문명: The Significance of Acetylcholine Receptor Autoantibody Test
• 발행기관: 대한핵의학기술학회
• 저자명: 유소연(Soh Yeon Yoo) 임수연(Soo Yeon Lim) 백송란(Song Ran Pack) 서미혜(Mi Hye Seo) 문형호(Hyung Ho Moon) 유선희(Sun Hee You)
• 간행물 정보: 『핵의학기술』Vol.15 No.1, 113~116쪽, 전체 4쪽
• 주제분류: 의약학 > 방사선과학
• 파일형태: PDF
• 발행일자: 2011.04.30

영문 초록

Purpose: Acetylcholine receptor antibodies cause acetylcholine receptor loss, which is responsible for failure of the neuromuscular junction in the acetylcholine receptor autoantibody. The disease characterized by muscle weakness and fatigue, myasthenia gravis(MG) occurs when the body inappropriately produces antibodies against acetylcholine receptors, and thus inhibits proper acetylcholine signal transmission. And this reason, the measurement of acetylcholine receptor antibodies can be of considerable value in disease diagnosis. Methods: From 2010. August to September, we tested orderd AchRAb 19 samples to get the results. 1. Pipette 5 ㎕ undiluted patient sera and kit control and add 125I AChR 50 ㎕ and incubate at R.T for 2 hours. 2. Pipette 50 ㎕ of anti-human IgG into each tube, and incubate at 2~8℃ for 2 hours. 3. Pipette 25㎕ precipitation enhancer into each tube and add 1mL washing solution into all tubes. 4. Centrifuge each tube for 20minutes at 2~8℃ at 1500g. 5. Aspirate or decant the supernatant. 6. Pipette 1 mL washing solution into all tubes and resuspend the pellet and repeat centrifugation. 7. Aspirate or decant the supernatant and count all tubes on a gamma counter. Results: Cut off value is 0.2 nmol/L and the results taken below 0.2 nmol/L are negative, the results above that identified as being positive values. We assayed the 19 patients samples and got 7 positive results. Of which, 6 patients were diagnosed as MG.(85.7%). Conclusions: Acetylcholine Receptor autoantibody test is intended for use by persons only for the quantitative determination of it in human serum. Even if measurement of the antibodies is not a routine test, it can be of considerable value in disease diagnosis.

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키워드

Acetylcholine Receptor autoantibody(AChRAb) Myasthenia gravis(MG).