소변 시료 중 메스암페타민 및 암페타민 광학이성질체 농도 측정 결과의 불확도 평가

• 영문명: Uncertainty Evaluation for Determination of Methamphetamine and Amphetamine Enantiomers in Urine
• 발행기관: 대한약학회
• 저자명: 김진영(Jin Young Kim) 심영은(Yeong Eun Sim) 고범준(Beom Jun Ko)
• 간행물 정보: 『약학회지』제63권 제6호(2019년), 374~383쪽, 전체 10쪽
• 주제분류: 의약학 > 기타의약학
• 파일형태: PDF
• 발행일자: 2019.12.30

영문 초록

The objective of this study was to assess the measurement uncertainty for determination of methamphetamine and amphetamine enantiomers in urine sample by liquid chromatography-tandem mass spectrometry (LC-MS/MS). The deuterium-labeled compounds were prepared for use as internal standards to quantify the analytes. Urine sample (200 μl) was diluted with pure water before the solid phase extraction (SPE). The sample was extracted and purified using Oasis HLB SPE cartridge followed by chromatographic separation on a vancomycin-based chiral column (150 mm×2.1 mm, 5 μm). The mass spectrometric analysis were performed in multiple reactions monitoring (MRM) selecting three precursor/ fragment ion pairs for each analyte. The potential sources that contribute uncertainty to the final result were evaluated. The major contributing factors impacting the combined standard uncertainty were calibration curve and repeatability, while the preparation of standard solution and sample dilution were minor factors. Relative expanded uncertainty of the measured analyte concentration was within 8.5% in a forensic urine sample.

목차

서 론(Introduction)

실험 방법(Experimental Methods)

결과 및 고찰(Results and Discussion)

결 론(Conclusion)

Conflict of Interest

References

키워드

Measurement uncertainty urine analysis enantiomer methamphetamine amphetamine LC-MS/MS