초고성능액체크로마토그래피-탠덤매스에 의한 혈장과 담즙 중 콜릭산의 정량 분석에 관한 연구

• 영문명: Analytical Method Development of Cholic Acid in Human Plasma and Gall Bladder Bile by Ultra Performance Liquid Chromatography-Tandem Mass Spectrometry
• 발행기관: 대한약학회
• 저자명: 나승훈(Seonghoon Na) 윤혜란(Hye-Ran Yoon)
• 간행물 정보: 『약학회지』제63권 제6호(2019년), 351~358쪽, 전체 8쪽
• 주제분류: 의약학 > 기타의약학
• 파일형태: PDF
• 발행일자: 2019.12.30

영문 초록

Bile acids are increasingly appreciated as bioactive molecules and important end products of cholesterol metabolism. While they have been identified as key factors in lipid emulsification and absorption due to their detergent properties. bile acids have also been shown to act as signaling molecules and intermediates between the host and the gut microbiota. To investigate bile acid functions in humans, an advanced platform for high throughput analysis is essential. Herein, we developed the analytical method of cholic acids following simple one step protein precipitation from biological sample by UPLC-MS/MS. MRM ions was m/z=407.2 for cholic acid. The R2 of calibration curves provided 0.9995 in the calibration range of 0.005~5 μg/mL. The quantification system was validated with excellent sensitivity that allows quantitative targeted analyses of bile acids. The LOD (0.001 μg/mL), LOQ (0.005 μg/mL), recoveries (96.8~101.3%± 2.7~4.0%) on intra-day assay, and (98.4~111.0%±2.3~3.4%) on inter-day assay achieved resonable validated data for bile acids analyses. The developed method was applied for drawing plasma and bile acid profile in both normal and disease status. Our study is characterized by rapid and simple sample preparation as well as successful application to plasma and bile. These results could be usable for routine diagnostic monitoring of bile acids on human biofluids.

목차

서 론(Introduction)

실험방법 (Experimental Method)

결 과(Results)

고 찰(Discussion)

결 론(Conclusion)

감사의 말씀(Acknowledgment)

Conflict of Interest

References

키워드

bile acids cholic acid stable isotope deproteinization UPLC-MS/MS plasma gall bladder