Chromatographic Enantiomer Separation of Chiral Amines as Fluorene-2-carboxaldimine Derivatives Using Polysaccharide-derived Chiral Stationary Phases

• 발행기관: 대한약학회
• 저자명: Suraj Adhikari Kwang Joon Kim Wonjae Lee
• 간행물 정보: 『약학회지』제63권 제6호(2019년), 328~332쪽, 전체 5쪽
• 주제분류: 의약학 > 기타의약학
• 파일형태: PDF
• 발행일자: 2019.12.30

영문 초록

Fluorene-2-carboxaldehyde was used as a new derivatizing agent for the first time to separate the enantiomers of chiral aliphatic amines including amino alcohols by normal phase HPLC under ultraviolet detection. The enantiomer separation of four chiral amines as fluorene-2-carboxaldimine derivatives was performed on six covalently bonded and four coated type polysaccharide-derived chiral stationary phases (CSPs) of amylose or cellulose-based chiral sorbents and the obtained chromatographic results were compared. The nature of the employed CSP (chiral selector or CSP type) and the used analyte type (aliphatic amines and amino alcohols) have significant impact on chromatographic parameters of enantioseparation and retention factor. In general, Chiralpak IE and Chiralpak IF showed good enantioseparation for all the analytes. In particular, the cellulose-derived CSPs showed the greatest enantiomer separation especially for amino alcohol analytes. Among the cellulose-derived CSPs, the coated type CSPs (Chiralcel OD-H and Lux Cellulose-1) showed better enantioselectivity and resolution for these analytes than the covalently bonded Chiralpak IB.

목차

Introduction

Experimental Methods

Results and Discussion

Conclusion

Acknowledgment

Conflict of Interest

References

키워드

Chiral amine chiral selector chiral stationary phase enantiomer separation fluorene-2-carboxaldimine derivative