대장균내 발현벡터의 안정성 향상으로 L-페닐알라닌 생산

• 영문명: Plasmid Stability and L-Phenylalanine Production in Escherichia Coli
• 발행기관: 한국산업기술융합학회(구. 산업기술교육훈련학회)
• 저자명: 고하영(Ha-Young Koh)
• 간행물 정보: 『산업기술연구논문지』산업기술연구논문지 제23권 4호, 49~55쪽, 전체 7쪽
• 주제분류: 공학 > 공학일반
• 파일형태: PDF
• 발행일자: 2018.12.30

영문 초록

The stability and L-phenylalanine productivity of a low-copy-number plasmid pSY200-14, in Escherichia coli AT2471, were investigated in batch culture. The pSY200-14 plasmid was stable in batch culture between 30℃ and 40℃ and L-phenylalanine productivity was shown similar to or higher than that of the high-copy-number plasmid pSY130-14. It was shown that the plasmid stability and L-phenylalanine production were regulated by temperature. Maximum yield of L-phenylalanine, 147㎎/ℓ was obtained using low-copy-number plasmid pSY200-14 in AT2471 at 38.5℃. The host strain AT2471 with pSY130-14, produced 100㎎/ℓ of L-phenylalanine at the same temperature. In this study, the L-phenylalanine synthesis enzyme genes, aro FFR and phe AFR, controlled by a temperature sensitive repressor, cI857. were cloned into the low-copy-number plasmid pSY200-14. In conclusion, plasmid stability and optimum L-phenylalanine production were constantly achieved in batch culture at high temperature.

목차

Ⅰ. 서 론

Ⅱ. 실험재료 및 방법

Ⅲ. 실험결과 및 고찰

Ⅳ. 결 론

키워드

Plasmid(플리스미드) Promoter(프로모터) L-Phenylalanine(L-페닐알라닌) aroF(방향족 유전자 F) Feedback Repression(피드백 억제)