돼지 흉막폐렴의 진단을 위한 효소면역법의 개발

• 영문명: Development and evaluation of an enzyme-linked immunosorbent assay for serodiagnosis of actinobacillus pleuropneumoniae infection in pigs
• 발행기관: 한국예방수의학회
• 저자명: 김봉환(Bong Bwan Kim)
• 간행물 정보: 『Korean Journal of Veterinary Public Health』Vol.22, No.1, 57~63쪽, 전체 7쪽
• 주제분류: 의약학 > 면역학
• 파일형태: PDF
• 발행일자: 1998.03.30

영문 초록

Capsular polysaccharides(CPS) of serotypes 1, 2 & 5 of Acinobacillus pleuropneumonie were prepared from 18 hr culture supernatants by precipitaion with hexadecyltrimethyl-ammonium bromide(Cetavlon) followed by extraction with sodium chloride, reprecipitation in ethyl alcohol and phenol extraction. The CPS were evaluated as antigens for the detection of serotype-specific antibodies to A. pleuropneumoniae in pig sera by an enzyme-linked immunosorbent assay(ELISA). Serotype-specific reactions were detected in pig sera with CPS antigens of serotype 1 and 5 but serotype 2 CPS antigen was slightly cross-reactive to serotype 5 antisenrum. The herds classified as posi-tive or chronically infected to A. pleuropneumoniae were readily identified by the ELISA, with all sampled pigs showing high levels of reactivity, while the clinically negative herds were found to be ngative. In conclusion, diagnosis of pleuropneumonia caused by A. pleuropneumoniae serotypes 1, 2 & 5 can be made by screening for antibodies using CPS antigen in ELISA.

목차

ABSTRACT

서론

재료 및 방법

결과

고찰

결론

참고문헌

키워드

Actinobacillus pleuropneumoniae Enzyme-linked immunosorbent assay Serodiagnosis Pigs