Marker Development for Onion Genetic Purity Testing using SSR Finder

• 발행기관: 한국육종학회
• 저자명: Hyoun-Joung Kim Heung-Ryul Lee Ji Young Hyun Ki Hyeon Song Kyu-Hyun Kim Jung Eun Kim Cheol-Goo Hur Chee Hark Harn
• 간행물 정보: 『한국육종학회지』Vol.44 No.4, 421~432쪽, 전체 12쪽
• 주제분류: 농수해양 > 기타농수해양
• 파일형태: PDF
• 발행일자: 2012.12.30

영문 초록

Molecular genetic markers have been widely used as powerful tools for analyzing the genome. In particular, SSR markers have practical applications in breeding systems because they can be used in high-throughput analyses for genetic mapping, heritable diversity testing, purity analysis, and marker-assisted selection. Currently, due to technical advances in DNA sequencing, large sequence databases are available for large-scale SSR mining and marker development. Here, we describe an automated method, the SSR Finder program, for SSR discovery in the onion sequence database, and primer design for amplifying the detected SSRs. A total of 1,049 SSR primers were obtained for genetic purity testing, and 100 SSR sets were analyzed in 14 bulb onion breeding lines using clustering analysis. A total of 20 selected markers from screening of all 1,049 SSR primers, were finally applied for genetic purity testing in three breeding lines, NW1, NW9, and NW10. The initial tests showed that 15%, 71%, and 97% of individuals within NW1, NW9, and NW10, respectively, were genetically homogeneous. These markers produced using the SSR Finder will be useful for investigating the genetic purity of onion breeding lines.

목차

INTRODUCTION

MATERIALS AND METHODS

RESULTS

DISCUSSION

키워드

clustering analysis genetic purity testing onion SSR marker SSR Finder