보통 밀에서 i-type 저분자글루테닌 유전자 클로닝 및 동정

• 영문명: Characterization of Three i-type Low-Molecular-Weight Subunit Genes in Common Wheat
• 발행기관: 한국육종학회
• 저자명: 이종열(Jong-Yeol Lee) 오기광(Ki-Kwang Oh) 김효정(Hyo-Jung Kim) 김영태(Yeong-Tae Kim) 강천식(Chon-Sik Kang) 지원재(Won-Jae Chi) 임선형(Sun-Hyung Lim) 하선화(Sun-Hwa Ha) 안상낙(Sang-Nag Ahn) 김영미(Young-Mi Kim)
• 간행물 정보: 『한국육종학회지』Vol.44 No.4, 567~578쪽, 전체 12쪽
• 주제분류: 농수해양 > 기타농수해양
• 파일형태: PDF
• 발행일자: 2012.12.30

영문 초록

Three low molecular weight (LMW) glutenin subunit genes were isolated from hexaploid wheat cultivars (Triticum aestivum. L) using LMW-GS specific primers and designated as Jokyung II-2, CS III-5 and Keumkang 6-12, respectively. All sequences were classified as LMW-i type genes based on the presence of an N-terminal isoleucine residue. All comprised of four regions, the signal peptide, N- and C- terminal domains, and a repetitive domain. Although they showed high similarity with other LMW-i type genes from hexaploid bread wheat, they also displayed unique features. Particularly, Keumkang 6-12 subunit contained an extra cysteine residue in the C-terminus except for eight conserved cysteines, which resulted from a single-nucleotide polymorphism (SNP) of the A–G transition, namely tyrosine-cysteine substitution at position 335 from the N-terminal end. In addition, a total of 15 SNPs and one insertions/deletions (InDels) were detected among Jokyung II-2, CS III-5 and Jokyung HQ6199333 and 10 other LMW-i genes. Two dimensional gel electrophoresis and LC-ESI MS/MS analysis revealed that LMW i-type glutenin subunit of the Jokyung is Glu-A3c. The phylogenic analysis showed that LMW i-type genes were divided roughly into diploid and hexaploid groups and the hexaploid were further classified into 7 subgroups (Glu-A3a~g). We also found length polymorphism in the repetitive domain showing 2~179 amino acid residues variation among the previously published LMW-i type glutenin subunits. The length polymorphism can be used as reliable genetic marker for use in marker-assisted breeding to select for specific alleles of the quality-determining locus in wheat breeding program.

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키워드

Wheat storage protein Glutenin LMW i-type subunits Wheat quality improvement