DNA 마커를 이용한 야생 복숭아 유전자원의 유전적 다양성 분석

• 영문명: Analysis of Genetic Diversity among Wild Peach Germplasms using DNA Markers
• 발행기관: 한국육종학회
• 저자명: 조강희(Kang Hee Cho) 남은영(Eun Young Nam) 배경미(Kyung-Mi Bae) 신일섭(Il Sheob Shin) 김세희(Se Hee Kim) 김현란(Hyun Ran Kim) 정경호(Kyeong Ho Chung)
• 간행물 정보: 『한국육종학회지』Vol.44 No.4, 588~596쪽, 전체 9쪽
• 주제분류: 농수해양 > 기타농수해양
• 파일형태: PDF
• 발행일자: 2012.12.30

영문 초록

In this study, sequence-related amplified polymorphism (SRAP), amplified fragment length polymorphism (AFLP), and randomly amplified polymorphic DNA (RAPD) analyses were used to assess genetic diversity among 64 peach germplasms including commercial cultivars and wild accessions. Nineteen SRAP primer combinations were detected a total of 143 polymorphic bands (26.7%) with an average 7.53. In AFLP analysis, twenty primer combinations were generated a total of 193 polymorphic bands (27.2%) with an average 9.65. Fifty-two RAPD primers were detected a total of 201 polymorphic bands (45.9%) with an average of 3.90. By UPGMA (unweighted pair-group method arithmetic average) cluster analysis with 537 polymorphic bands, the peach germplasms were classified eight groups by similarity index of 0.751. Genetic similarity values among the tested peach germplasms ranged from 0.370 to 0.978, and the average similarity value was 0.737. The similarity index was the highest (0.978) between PH065 and PH066, and the lowest (0.370) between Prunus davidiana and PH020. The genetic diversity among the 64 peach germplasms revealed by cluster analysis was low, despite they were collected from different regions.

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키워드

Peach AFLP RAPD SRAP marker Genetic diversity