비타민A 강화벼의 환경위해성 평가 : 4세대의 후대안정성 연구

• 영문명: Evaluation and Assessment of Biosafety Carotenoid-biofortified Rice (PAC) : Progeny Stability Study for Four Generations
• 발행기관: 한국육종학회
• 저자명: 오성덕(Sung-Dug Oh) 박수윤(Soo-Yun Park) 손수인(Soo-In Sohn) 김재광(Jae-Kwang Kim) 박종석(Jong-Sug Park) 박순기(Soon Ki Park) 조현석(Hyun Suk Cho) 안병옥(Byung-Ohg Ahn) 이기종(Kijong Lee)
• 간행물 정보: 『한국육종학회지』Vol.44 No.4, 579~587쪽, 전체 9쪽
• 주제분류: 농수해양 > 기타농수해양
• 파일형태: PDF
• 발행일자: 2012.12.30

영문 초록

The carotenoid-biofortified (PAC) rice was developed and confirmed to synthesis carotenoids (zeaxanthin, α-carotene and β-carotene) in rice endosperm. For biosafety assessment of PAC rice, the stability of the insert genes in carotenoid-biofortified rice at genomic and protein expression level through different generations (T3~T6) was studied. Southern blot analysis confirmed that the multi-generation stability of the genes : PAC gene (CrtI and Psy), promoters (35S and Glb), terminators (NOS and PinII), selectable marker (bar) and MAR gene. It was also confirmed that the backbone DNA containing antibacterial gene (aadA) was not present in PAC rice. Immuno-strip assay (PAT) showed that the bar gene was stably expressed through generations. HPLC analysis confirmed that carotenoids were consistently detected through T3~T6 generations. These results will be filed to biosafety assessment document of PAC rice.

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키워드

Biosafety Transgenic rice β-Carotene Progeny study