고초균 유래 2-디옥시리보오스 5-포스페이트 알돌라아제 유전자(deoC )의 클로닝, 발현 및 특성연구

• 영문명: Cloning, Expression and Characterization of 2-Deoxyribose Phosphate Aldolase Gene(deoC) from Bacillus sp.168
• 발행기관: 한국산업기술융합학회(구. 산업기술교육훈련학회)
• 저자명: 정성오(Sung-O h C hung)
• 간행물 정보: 『산업기술연구논문지』산업기술교육훈련논문지 제18권 3호, 99~103쪽, 전체 5쪽
• 주제분류: 공학 > 공학일반
• 파일형태: PDF
• 발행일자: 2013.09.30

영문 초록

A deoC gene encoding 2-Deoxyribose phosphate aldolase(DERA) was cloned from Bacillus subtilis 168 into an expression vector and protein was expressed in Escherichia coli. A molecular weight of the expressed recombinant DRRA was approximately 24.5kDa. The recombinant DRRA was purified using Ni-NTF affinity chromatography and then characterized. The degree of purification yield of the purified recombinant DERA were 1.4-fold and 74%, respectively, compared with that of the crude extract. The optimum temperature and pH of the enzyme were 20∼50℃ and pH6.0, respectively. The thermostability of the enzyme showed that more than 42% activity remained after at 40℃ for 24h, but no activity over 60℃ at the same condition.

목차

Ⅰ. 서 론

Ⅱ. 실험재료 및 방법

Ⅲ. 실험결과 및 고찰

Ⅳ. 결 론

키워드

deoC DERA유전자 Bacillus subtilis 바실러스 서버틸러스 2-Deoxyribose Phosphate Aldolase 2-디옥시리보오스 5-포스페이트 알돌라아제