히스티딘-리치 Ca2+ 결합 단백질에 의한 심실근세포 수축 및 Ca2+ Transient의 조절

• 영문명: Regulation of Contraction and Ca2+ Transient by Histidine-rich Ca2+-binding Protein in Ventricular Myocytes
• 발행기관: 대한약학회
• 저자명: 손민정(Min-Jeong Son) 김준철(Joon-Chul Kim) 김성우(Seong-Woo Kim) 안종렬(Jong-Real Ahn) 우선희(Sun-Hee Woo)
• 간행물 정보: 『약학회지』제56권 제6호 (2012년), 377~381쪽, 전체 5쪽
• 주제분류: 의약학 > 기타의약학
• 파일형태: PDF
• 발행일자: 2012.12.31

영문 초록

The histidine-rich Ca2+ binding protein (HRC) is a Ca2+ binding protein in the sarcoplasmic reticulum (SR). In this study, we examined whether the HRC is involved in the regulation of cardiac contraction and Ca2+ signaling using HRC knock-out (KO) mouse ventricular myocytes. In field-stimulated single mouse ventricular myocytes, cell shortenings and Ca2+ transients were measured using a video edge detection and a confocal Ca2+ imaging, respectively. Compared with the wide-type (WT) myocytes, the magnitudes of cell shortenings were significantly larger in HRC KO cells (P<0.01, WT vs. KO). The rate of contraction and relaxation was significantly accelerated in HRC KO myocytes (P<0.05 and P<0.01, respectively, WT vs. KO). The magnitudes of Ca2+ transients were increased by HRC KO (P<0.01, WT vs. KO). In addition, the decay of the Ca2+ transient was faster in HRC KO cells than in wild-type cells P<0.01, WT vs. KO). These results suggest that HRC may suppress SR Ca2+ releases and decay of Ca2+ transients during action potentials, thereby attenuating ventricular contraction and relaxation.

키워드

histidine-rich Ca2+ -binding protein (HRC) contraction Ca2+ transient ventricular myocytes knock-out