3T3-L1 세포에서 소맥엽 에탄올추출물의 지질생성 억제효과

• 영문명: Inhibitory Effect of Triticum aestivum Ethanol Extract on Lipid Accumulation in 3T3-L1 Preadipocytes
• 발행기관: 대한약학회
• 저자명: 이선희(Sun-Hee Lee) 신명걸(Mingjie Xin) Bui Thi Thuy Luyen 차지윤(Ji-Yun Cha) 임지영(Ji-Young Im) 권세욱(Se-Uk Kwon) 임성원(Sung-Won Lim) 서주원(Joo-Won Suh) 김영호(Young-Ho Kim) 김대기(Dae-Ki Kim) 이영미(Young-Mi Lee)
• 간행물 정보: 『약학회지』제55권 제6호 (2011년), 478~484쪽, 전체 7쪽
• 주제분류: 의약학 > 기타의약학
• 파일형태: PDF
• 발행일자: 2011.12.31

영문 초록

Non-alcoholic fatty liver disease is known to be frequently associated with obesity and type 2 diabetes. We examined the effects of EtOH extracts from Triticum aestivum on lipid accumulation during the differentiation of 3T3-L1 preadipocytes to screening the candidate materials in preventing non-alcoholic fatty liver disease. The lipid level in adipocytes was determined by Oil Red O staining. The treatment of 50% ethanol, but not water and 100% ethanol extracts, from Triticum aestivum at concentration of 0.5 mg/ml inhibited lipid accumulation in 3T3-L1 cells, revealing no cell toxicity. Thus, the fractions of CH2Cl2, EtOAc and BuOH were separated from 50% EtOH extract to characterize anti-adipogenic effect. The CH2Cl2 fraction at concentration of 50 μg/ml effectively inhibited the lipid accumulation in the adipocytes compared to those of EtOAc and BuOH at concentration of 50 μg/ml. The intracellular triglyceride accumulation also was significantly reduced by treatment of CH2Cl2 fraction in concentration-dependent manner. Western blot analysis showed that the CH2Cl2 fraction attenuated the intracelluar level of fatty acid synthase(FAS) accompanied by attenuated expression of Peroxidase proliferator-activated receptor γ (PPARγ) adipogenic transcription factor. These results suggest that CH2Cl2 fraction from 50% EtOH extract of Triticum aestivum may has the potent anti-adipogenic effects by inhibiting the transactivation of PPARγ.

키워드

Triticum aestivum 3T3-L1 preadipocytes lipid accumulation fatty acid synthase anti-adipogenesis