메칠글라이옥살이 섬유주세포의 산화스트레스에 미치는 영향

• 영문명: Effect of Methylglyoxal on the Oxidative Stress in Trabecular Meshwork Cells
• 발행기관: 대한안과학회
• 저자명: 이승희 김신후 김재우,Seung Hee Lee, MD, Sin Hoo Kim, MD, Jae Woo Kim, MD, PhD
• 간행물 정보: 『대한안과학회지』Ophthalmological Society,volume50,number10, 1569~1575쪽, 전체 7쪽
• 주제분류: 의약학 > 기타의약학
• 파일형태: PDF
• 발행일자: 2009.10.15

영문 초록

Purpose: To investigate the effect of methylglyoxal (MG), intermediate metabolite of advanced glycation end products(AGE), on the induction of oxidative stress in human trabecular meshwork cells (HTMC). Methods: Primarily cultured HTMC were exposed to at concentrations of 0, 30, 100, and 300 μM of MG for 18 hours, with or without co-exposure to N-acetyl-cysteine. Cellular survival and apoptosis were assessed by MTT assay and flow cytometry using annexin-PI double staining. Production of nitric oxide (NO), superoxide, and reactive oxygen species (ROS) was assessed by Griess assay, cytochrome c assay, and dichlorofluorescein diacetate assay, respectively. Results: MG did not affect cellular survival at concentrations under 100 μM, but induced apoptosis of HTMC at concentrations over 100 μM. MG decreased NO production, accompanied with increased superoxide production. In addition, MG increased ROS, which were abolished by N-acetylcysteine. Conclusions: MG induced oxidative stress by decreasing NO production, accompanied by increasing superoxide and ROS productions in HTMC. AGE could induce trabecular meshwork dysfunction.

키워드

Advanced glycation end products, Methylglyoxal, Oxidative stress, Trabecular meshwork cells