고포도당이 섬유주세포의 산화스트레스에 미치는 영향

• 영문명: Effect of High Glucose on the Oxidative Stress in Trabecular Meshwork Cells
• 발행기관: 대한안과학회
• 저자명: 김재우 배창범 이정훈,Jae Woo Kim, MD, PhD, Chang Beum Bae, MD, Jeong Hun Lee, MD
• 간행물 정보: 『대한안과학회지』Ophthalmological Society,volume50,number10, 1563~1568쪽, 전체 6쪽
• 주제분류: 의약학 > 기타의약학
• 파일형태: PDF
• 발행일자: 2009.10.15

영문 초록

Purpose: To investigate the effect of high glucose (HG) on the oxidative stress in cultured human trabecular meshwork cells (HTMC). Methods: Primarily cultured HTMC were exposed to low glucose (5 mM) and HG (25 mM) for 7 days. Additionally, 1 mM Larginine, 5 mM DAHP, 10 μg/ml insulin, 100 μM L-ascorbic acid, 10, and 100 μM sepiapterin were co-exposed. The cellular survival and nitric oxide (NO) production were assessed by MTT assay and Griess assay, respectively. Superoxide production was measured by modified cytochrome c assay. Results: HG did not affect the survival of cultured HTMC significantly. HG decreased NO production. Co-exposed DAHP decreased but DAHP and insulin increased NO production. In addition, HG increased superoxide production, which was decreased by insulin, L-ascorbic acid, and sepiapterin. Conclusions: HG decreased NO production accompanied with increased superoxide production in HTMC. Thus HG induces oxidative stress in HTMC and may cause cellular dysfunction and damage of the trabecular meshwork.

키워드

Glucose, Nitric oxide, Oxidative stress, Superoxide, Trabecular meshwork cells