포도당 농도가 인간 수정체상피세포의 반응성 산소 생성량의 변화에 미치는 영향

• 영문명: The Effects of Glucose Concentrations on Reactive Oxygen products in Human Lens Epithelial Cells
• 발행기관: 대한안과학회
• 저자명: 양유리 이병래 고재웅,Yoo Ri Yang, M.D., Bypung Rai Lee, M.D., Jae Woong Koh, M.D.
• 간행물 정보: 『대한안과학회지』Ophthalmological Society,volume47,number5, 828~833쪽, 전체 6쪽
• 주제분류: 의약학 > 기타의약학
• 파일형태: PDF
• 발행일자: 2006.05.31

영문 초록

Purpose: To investigate the effects of glucose concentrations on the formation of reactive oxygen products and cellular injury in human lens epithelial (HLE) cells. Methods: Human lens epithelial (HLE) cells were cultured with high glucose (200 mg/100 ml, 300 mg/100 ml, 400 mg/100 ml) and normal glucose (100 mg/100 ml) contained Modified Egle`s Medium (MEM). The amounts of reactive oxygen products were assayed by dihydroethidium (DHE). Paraquat-induced cytotoxicity was determined by the 3-[4,5-dimethylthiazol-2-yl]-2,5 -diphenyl tetrazolium bromide (MTT) method. Results: Reactive oxygen products of human lens epithelial cells increased 110%, 250% and 630% in the high glucose (200 mg/100 ml, 300 mg/100 ml, 400 mg/100 ml) media compared to the normal glucose (100 mg/100 ml) media. The paraquat-induced cells toxicity was increased by the high glucose. Conclusions: High glucose increased the formation of reactive oxygen products in human lens epithelial cells. These results suggest that high glucose can induce cataract formation in diabetic patients.

키워드

High Glucose, Human Lens Epithelial Cells, Reactive Oxygen