당뇨쥐의 각막실질세포에서 세포자멸사와 관련된 유전자 연구

• 영문명: Gene Microarray Related with Apoptosis in Diabetic OLETF Keratocytes
• 발행기관: 대한안과학회
• 저자명: 이지은 김해송 이종수,Ji Eun Lee, M.D., Hae Song Kim, M.D., Jong Soo Lee, M.D.
• 간행물 정보: 『대한안과학회지』Ophthalmological Society,volume47,number5, 818~827쪽, 전체 10쪽
• 주제분류: 의약학 > 기타의약학
• 파일형태: PDF
• 발행일자: 2006.05.31

영문 초록

Purpose: This study was to identified differentiated genes concerned with apoptosis that are up-regulated or down-regulated in OLETF corneal keratocytes stimulated with interleukin-1α. Methods: Otsuka Long Evans Tokushima Fatty (OLETF) corneal keratocytes were primarily cultured and treated with 20 ng/ml of IL-1a for 6 hours. Hybridization was carried out using Oligonucleotide microarray. A sample of normal keratocytes was labeled with Cy 3, and a diabetic keratocytes sample was labeled with Cy 5. Results: Diabetes showed 20 down-regulated genes and 24 up-regulated genes compared with normal keratocytes. Also, IL-1α-treated diabetic keratocytes expressed 31 down-regulated and 33 up-regulated genes. Compared with diabetic keratocytes, the newly expressed genes in OLETF treated with IL-1α were Bcl, Lam, Timp, Mmp, Socs, Bmp, Ccl, Lcn, C7, etc. Conclusions: There were some genes related with apoptosis that expressed differently between normal and diabetic keratocytes of OLETF. Also, IL-1α may stimulate differing effects of apoptosis on diabetic corneal keratocytes. Studies analyzing the apoptotic significance of these differences identified in diabetic keratocytes are needed.

키워드

Apoptosis, Diabetes mellitus, Keratocyte, Microarray, Rat