B16F10 Murine Melanoma 세포에서 멜라닌생성억제에 대한 타우린 효과

• 영문명: Antimelanogenic Effect of Taurine in Murine Melanoma B16F10 Cells
• 발행기관: 대한약학회
• 저자명: 정효숙(Hyo Sook Joung) 송경희(Kyung Hee Song) 김안근(An Keun Kim)
• 간행물 정보: 『약학회지』제51권 제5호 (2007년), 350~354쪽, 전체 5쪽
• 주제분류: 의약학 > 기타의약학
• 파일형태: PDF
• 발행일자: 2007.10.31

영문 초록

Taurine has been shown to be tissue-protective against oxidant-induced injury and is a powerful regulator of the immune system. However, there is no study on the antimelanogenic effect of taurine. In this study, we investigated the whitening effect of taurine in B16F10 mouse melanoma cells. Cell viability was measured by MTT assay. We examined melanin contents and tyrosinase activity according to time and concentration. Extracellular signal regulated kinase (ERK) is an important regulator of melanogenesis. It has been reported that activated ERK induced microphthalmia associated transcription factor (MITF) phosphorylation and its subsequent degradation and thus reduced melanin synthesis. In our B16F10 cell culture system, taurine led to decrease melanin contents by 21% at 48 hr. We then observed taurine effects on ERK-P, MITF and tyrosinase by Western blot. ERK was activated at 18 hr and 24 hr, whereas MITF reduced. We could not observe any differences in the levels of tyrosinase. These results suggested that taurine inhibited melanogenesis by ERK signal pathway via MITF degradation. We expect that taurine has potential skin whitening agents in cosmetics.

키워드

taurine ERK MITF tyrosinase melanogenesis