인슐린 매개성 Microsomal Epoxide Hydrolase의 발현증가에서 Akt의 역할

• 영문명: Role of Akt in Insulin-Mediated Increase in Expression of Microsomal Epoxide Hydrolase
• 발행기관: 대한약학회
• 저자명: 김상겸(Sang Kyum Kim) 김봉희(Bong Hee Kim) 오정민(Jung Min Oh) 윤강욱(Kang Uk Yun) 김충현(Chung Hyeon Kim) 강건욱(Keon Wook Kang)
• 간행물 정보: 『약학회지』제51권 제5호 (2007년), 291~295쪽, 전체 5쪽
• 주제분류: 의약학 > 기타의약학
• 파일형태: PDF
• 발행일자: 2007.10.31

영문 초록

The present study examines the effect of dominant-negative Akt on the insulin-mediated microsomal epoxide hydrolase (mEH) induction in rat hepatocytes. We also assessed the role of insulin in the expression of soluble epoxide hydrrolase (sEH). Insulin increased mEH levels and the enzyme activities, whereas sEH protein expression was unaffected by insulin. The specific PI3K inhibitors or p70 S6 kinase inhibitor ameliorated the insulin-mediated increase in mEH protein levels. Infection with adenovirus expressing dominant-negative and kinase-dead mutant of Akt1 effectively inhibited the insulin-mediated increase in mEH expression and mEH activity. These results suggest that mEH and sEH are differentially regulated by insulin and PI3K/Akt/p70S6K are active in the insulin-mediated regulation of mEH expression.

키워드

microsomal epoxide hydrolase soluble epoxide hydrolase insulin cellular signaling pathway akt