생기액(生肌液)의 세포독성 및 자궁경부암 바이러스 (HPV 16 type) 암 유발인자 E6와 E7의 작용에 미치는 효과

• 영문명: The Effects of Somatid on the Cytotoxicity of Cancer Cells and Human Papillomavirus Type 16 E6 and E7 Oncogenes
• 발행기관: 대한약학회
• 저자명: 정옥(Ok Joung) 조영식(Young Sik Cho) 조정원(Cheong Weon Cho) 이경애(Kyung Ae Lee) 심정현(Jung Hyun Shim) 조민철(Min Chul Cho) 이홍수(Hong Soo Lee) 염영일(Young Il Yeom) 김상범(Sang Bom Kim) 박순희(Sue Nie Park) 윤도영(Do Young Yoon)
• 간행물 정보: 『약학회지』제44권 제4호 (2000년), 340~346쪽, 전체 7쪽
• 주제분류: 의약학 > 기타의약학
• 파일형태: PDF
• 발행일자: 2000.08.30

영문 초록

Cervical cancer is one of the leading causes of female death from cancer worldwide with about 500,000 deaths per year. A strong association between certain human papilloma viruses (HPV types 16 and 18) and cervical cancer has been well known. An extract of natural products, named as Somatid, has been used to investigate whether this agent has the ability of inhibiting the oncogenes E6 and E7 of HPV type 16. This Somatid inhibited the proliferation of human cervical cancer cell lines (C-33A, SiHa, CaSki) and HaCaT keratinocytes in a dose response manner. In vitro binding assay and ELISA showed that Somatid inhibited the in vitro biding of E6 and E6AP which are essential for the binding and degradation of the tumor suppressor p53. In addition, Somatid inhibited the in vitro binding of E7 and Rb which is essential tumor suppressor for the control of cell cycle. The levels of mRNA for E6 and E7 were also decreased by Somatid. Our data suggested that Somatid inhibited the oncogenecity of E6 and E7 of HPV 16 type, thus can be used as a putative anti-HPV agent for the treatment of cervical carcinomas caused by HPV.

키워드

In vitro binding assay Enzyme-linked Immunosorbent Assay(ELISA) E6 oncogene.