파상풍 톡소이드를 함유한 생체분해성 미립구의 특성

• 영문명: Characteristics of Tetanus Toxoid Loaded in Biodegradable Microparticles
• 발행기관: 대한약학회
• 저자명: 김지윤(Ji Yoon Kim) 김수남(Su Nam Kim) 백선영(Sun Young Baek) 이명숙(Myoung Sook Lee) 민홍기(Hong Ki Min) 홍성화(Seung Hwa Hong)
• 간행물 정보: 『약학회지』제44권 제4호 (2000년), 293~299쪽, 전체 7쪽
• 주제분류: 의약학 > 기타의약학
• 파일형태: PDF
• 발행일자: 2000.08.30

영문 초록

Biodegradable microspheres made from poly-lactide-co-glycolide polymers have been considered as a new delivery system for single-dose vaccine. Purified tetanus toxoid (TT) was encapsulated in poly-lactide(PLA) and poly-lactide-co-glycolide (PLGA) microparticles using a solvent evaporation method in a multiple emulsion system (water-in oil-in water). The morphology of TT-loaded microparticles was spherical and the surface of them was smooth. The particle size was in a range of 2-10. Protein loading efficiency was 68-97.8%. PLGA (85:15) microparticle showed the highest efficiency. Protein release pattern was influenced by polymer molecular weight and composition. The release rate of PLA (Mw 100,000) microsphere was higher than any other microspheres. In consequence of the hydrolysis of PLGA(50:50) microspheres, environmental pH decreased from 7.4 to 5.0. The PLA, PLGA (75:25) and PLGA (85:15) microspheres showed no significant pH change. The antigenicity of TT in microspheres was assayed by indirect sandwich ELISA using equine polyclonal tetanus antitoxin for capture antibody and human poly-clonal tetanus antitoxin for primary antibody. The antigenicity of TT in PLA (Mw 100,000), PLGA(50:50, Mw 100,000) and PLGA (75:25, Mw 73,300) after 30 days incubation showed 54, 40.9 and 76.7%, respectively.

키워드

Poly(lactic acid)(PLA) poly(lactic/glycolic acid)(PLGA) tetanus toxoid.