Brevibacterium lipolyticum 변이주에 의한 1,4-Androstadiene-3,17-Dione의 생성

• 영문명: Production of 1,4-Androstadiene-3,17-dione by a Mutant Strain of Brevibacterium lipolyticum
• 발행기관: 대한약학회
• 저자명: 최인화(In Wha Choi) 이강만(Kang Man Lee)
• 간행물 정보: 『약학회지』제33권 제6호 (1989년), 365~371쪽, 전체 7쪽
• 주제분류: 의약학 > 기타의약학
• 파일형태: PDF
• 발행일자: 1989.12.28

영문 초록

Microbiological conversion of sterols to 17-ketosteroids has been recognized as a source for commercial preparation of steroidal drugs. In order to develop bacterial strains and process with Brevibacterium lipolyticum IAM 1398 capable of converting cholesterol to 1,4-Androstadiene-3,17-dione (ADD) at about 27% yield, we studied on strain improvement, fermentation condition and whole cell immobilization. By using UV and/or NTG as mutagens, a mutant to convert cholesterol to ADD with higher yield than 60% was selected. Better production of ADD was manifested in the case of maltose used as a supplemental carbon source, and yeast extract or soytone as a nitrogen source. Addition of tween 80 (0.05%) as a surfactant beneficial for increasing the productivity. The optimal initial pH of the medium was 6.5 and optimal culture temperature was 30oC. Whole cell immobilization by using carrageenan, agar, alginate and acrylamide was carried out and the activity of conversion was tested. In the case of carrageenan and agar, immobilized cells were active for at least two cycles of fermentation.

키워드

Brevibacterium lipolyticum mutant ADD fermentation whole cell immobilization.