한국산 고등균류에 관한 연구(제 5 보) -능이 중 단백분해효소의 특성과 N-말단 아미노산배열-

• 영문명: Studies on Higher Fungi in Korea (V) -N-Terminal Amino Acid Sequence and Some Properties of Proteolytic Enzyme from Sarcodon aspratus-
• 발행기관: 대한약학회
• 저자명: 은재순(Jae Soon Eun) 양재헌(Jae Hean Yang) 이태규(Tae Kyu Lee) 최동성(Dong Seong Choi)
• 간행물 정보: 『약학회지』제33권 제6호 (1989년), 339~344쪽, 전체 6쪽
• 주제분류: 의약학 > 기타의약학
• 파일형태: PDF
• 발행일자: 1989.12.28

영문 초록

The alkaline protease produced by Sarcodon aspratus(Berk) S. Ito. was purified from its fruit bodies. The enzyme was purified by using ammonium sulfate fractionation, tris-acryl CM-cellulose column chromtography and chromatofocusing. The protease migrated as one major band with a molecular weight of about 29,000 dalton on sodium dodecylsulfate-polyacrylamide gel electrophoresis. The amino acid sequence of the N-terminal residues(21) of the enzyme was determined by automated sequence analysis. The sequence was Val-Thr-Thr-Lys-Gln-Thr-Asn-Ala-Pro-Trp-Gly-Leu-Gly-Asn-Ile-Ser-Thr-Thr-Asn-Lys-Leu. Comparison of this sequence with the N-terminal sequence of the p-roteinase K from Tritirachium album showed high similarity, i. e. 57.8% identical residues. The protease displayed a relatively high stability in sodium dodecyl sulfate.

키워드

Alkaline protease Sarcodon aspratus molecular weight N-terminal amino acid sequence.