기체 Chromatograph를 이용한 체액내 Volatile Free Acid의 분석연구

• 영문명: Volatile Free Acid Profiling of Body Fluids by Gas Chromatography
• 발행기관: 대한약학회
• 저자명: 김경례(Kyoung Rae Kim) 한미경(Mi Kyoung Hahn)
• 간행물 정보: 『약학회지』제32권 제1호 (1988년), 6~13쪽, 전체 8쪽
• 주제분류: 의약학 > 기타의약학
• 파일형태: PDF
• 발행일자: 1988.02.28

영문 초록

A gas chromatographic method is described for the determination of volatile free acids(VFAs) in body fluids. VFAs were trace enriched from body fluids by liquid-solid extraction using Chromosorb P as the solid sorbent and ether as the eluant. The enriched VFAs were injected in splitless injection mode onto a HP-20M fused silica capillary column. The flame ionization detector was used as the detector. The present method was applied to the profiling of VFAs in body fluids from patients suffering from the infectious disease, hepatitis. The VFAs concentrations were high in saliva of hepatitis patients and isobutyric acid detected in sera of hepatitis patients compared to healthy subjects.