RAW 264.7 대식세포에서의 유산균에 의한 Nitric Oxide와 TNF-α의 생성 증가 효과

• 영문명: Effect of Lactic Acid Bacteria (Lactobacillus acidophilus, Streptococcus thermophilus, Bifidobacterium bifidum) on the Enhancement of the Production of Nitric Oxide and TNF-α in RAW 264.7 Macrophage Cell
• 발행기관: 대한약학회
• 저자명: 박소희(So Hee Park) 정명준(Myung Jun Chung) 김수동(Soo Dong Kim) 백대헌(Dae Heoun Baek) 강병용(Nam Joo Ha) 하남주( Nam Joo Ha)
• 간행물 정보: 『약학회지』제49권 제6호 (2005년), 459~464쪽, 전체 6쪽
• 주제분류: 의약학 > 기타의약학
• 파일형태: PDF
• 발행일자: 2005.12.31

영문 초록

The immune reinforcement of the probiotic lactic acid bacteria Lactobacillus acidophilus, Streptococcus thermophilus and Bifidobacterium bifidum was studied in RAW 264.7 cell line treated with diluted solution (dilution to 25) of the supernatnats of lactic acid bacteria. RAW 264.7 cell line was used as a macrophage model to assess the effects of lactic acid bacteria on the production of nitric oxide (NO) and cytokine tumor necrosis factor (TNF)-α and cell morphological changes. The production of NO and TNF-α were largely affected by lactic acid bacteria in dose-dependent manner in 24 or 48 hr cultures and cell morphological changes were also largely affected by lactic acid bacteria. Especially, Bifidobacterium bifidum differentially stimulated the production of NO and TNF-α. NO production was increased by Bifidobacterium bifidum 25㎕/㎖ more than LPS(20ng/㎖) control, and TNF-α by Bifidobacterium bifidum 6.25㎕/㎖ more than LPS (10ng/㎖) contorl. The in vito approaches employed here should be useful in further characterization of the effects of lactic acid bacteria on systemic immunity.

키워드

lactic acid bacteria macrophage nitric oxide TNF-α