췌조직내 Nitric Oxide의 생합성

• 영문명: Biosynthesis of Nitric Oxide in Pancreatic Tissues
• 발행기관: 대한약학회
• 저자명: 김용기(Yong Kee Kim) 남석우(Suk Woo Nam) 박승희(Seung Hee Park) 유세근(Se Geun Yoo) 홍성렬(Sung Youl Hong) 이향우(Hyang Woo Lee)
• 간행물 정보: 『약학회지』제38권 제1호 (1994년), 24~30쪽, 전체 7쪽
• 주제분류: 의약학 > 기타의약학
• 파일형태: PDF
• 발행일자: 1994.02.28

영문 초록

Nitric oxide(NO) synthase was identified and characterized by determining the L-citrulline formed in the NO-Arg pathway in pancreatic tissues. NO synthase activities in chicken pancreas were dependent upon the concentration of L-Arg which is the substrate molecule for the NO synthase, the amount of the enzyme protein used, and linearly on the incubation time. NO synthase in mouse pancreas was found to be constitutive, not induced by lipopolysaccharide treatment. In vitro NO synthase activities of chicken pancreas were inhibited 36%, 21%, 12% and 44% by 200mcM of MMA, DMA, D''MA and NAME respectively. These results suggest the presence of NO and NO synthase in the pancreas.