Capsaicin 가수분해효소의 흰쥐 간세포내 소재확인

• 영문명: Subcellular Localization of Capsaicin-Hydrolyzing Enzyme in Rat Hepatocytes
• 발행기관: 대한약학회
• 저자명: 박영호(Young Ho Park) 이상섭(Sang Sup Lee)
• 간행물 정보: 『약학회지』제38권 제1호 (1994년), 12~19쪽, 전체 8쪽
• 주제분류: 의약학 > 기타의약학
• 파일형태: PDF
• 발행일자: 1994.02.28

영문 초록

Capsaicin(8-methyl-N-vanillyl-6-nonenamide) is the principal pungent component of Capsicum fruits. This work is directed to the capsaicin-hydrolyzing enzyme playing a key role in the rate limiting and critical step of capsaicin metabolism. In order to get precise information on the enzyme''s subcellular location, rat liver homogenate was divided into six subcellular fractions by differential centrifugation technique: crude nuclear pellet, PNS(post nuclear supernatant) fraction, lysosomal pellet, cytosol, Tris wash fraction, micrisomes. Capsaicin-hydrolysing enzyme activity was analysed by high performance liquid chromatography(HPLC). This enzyme was found at the highest specific activity in the microsomal fraction and co-distributed with marker enzymes of the endoplasmic reticulum, NADPH-cytochrome c reductase and nucleoside diphosphatase. This is compatible with the result of ninhydrin color reaction of vanillylamine, primary metabolite of capsaicin hydrolysis, on thin layer chromatography(TLC). This enzyme is most active at pH 8.0-9.0. Definite subcellular location of this enzyme will make it easy to proceed with further study.

키워드

Capsaicin-hydrolyzinK enzyme marker enzyme assay endoplasmic reticulum rat hepatocyte.