HPLC를 이용한 우유중의 클로람페니콜의 정량 분석

• 영문명: Determination of Chloramphenicol in Milk by High Performance Liquid Chromatography
• 발행기관: 대한약학회
• 저자명: 김경례(Kyoung Rae Kim) 김정한(Jung Han Kim) 최경숙(Kyoung Sook Choi)
• 간행물 정보: 『약학회지』제29권 제1호 (1985년), 50~54쪽, 전체 5쪽
• 주제분류: 의약학 > 기타의약학
• 파일형태: PDF
• 발행일자: 1985.02.28

영문 초록

Seven different sorbents were evaluated for their adsorptivity and desorptivity of antibiotic, chloramphenicol. Among the sorbents studied, Carbopak B was found to be the most efficient in enriching the chloramphenicol from dilute aqueous solution. Interfering components in the milk matrix could be washed off by water and petroleum ether from Carbopak B column, while the chloramphenicol was retained on the surface of Carbopak B. The method of simple and efficient purification and enrichment of chloramphenicol using Carbopak B, followed by quantitative analysis employing C18 reversed phase high performance liquid chromatography has been applied to the determination of chloramphenicol in milk.