인삼단백질분획에 대한 SDS-폴리 아크릴아미드 전기영동

• 영문명: SDS-polyacrylamide Gel Electrophoresis on Partially Purified Ginseng Proteins
• 발행기관: 대한약학회
• 저자명: 김춘미(Choon Mi Kim)
• 간행물 정보: 『약학회지』제27권 제4호 (1983년), 315~320쪽, 전체 6쪽
• 주제분류: 의약학 > 기타의약학
• 파일형태: PDF
• 발행일자: 1983.12.30

영문 초록

Ginseng proteins have been extracted and partially purified by the methods of ammonium sulfate fractionation, heat inactivation and Sephadex G-75 column chromatography. The final three fractions obtained (GI, GII and GIII) were subjected to paper chromatography and SDS-polyacrylamide gel electrophoresis. Molecular weights of polypeptide chains from each fraction were estimated by the standard line obtained from the plot of electrophoretic mobilities against the logarithm of molecular weights of standard proteins. Results are as follows: 1) GI showed three protein spots and four polypeptides of which M.W. were 63,000, 60,000, 56,000, 51,000 and 34,200. 2) GII showed four protein spots and five polypeptides with their M.W. of 64,600, 56,000, 45,400, 35,800, and 25,200. 3) GIII showed three protein spots and four polypeptides with their M.W. of 66,000, 63,000, 56,000 and 22,000.