고속 액체 크로마토그라피에 의한 cis- 및 trans-Neopynamin과 Fenitrothion의 분리 정량에 관한 연구

• 영문명: Studies on the Differential Determination of cis-, and trans-Neopynamin and Fenitrothion by High Pressure Liquid Chromatography
• 발행기관: 대한약학회
• 저자명: 이왕규(Wang Kyu Lee) 정해수(Hae Soo Chung)
• 간행물 정보: 『약학회지』제24권 제2호 (1980년), 117~122쪽, 전체 6쪽
• 주제분류: 의약학 > 기타의약학
• 파일형태: PDF
• 발행일자: 1980.06.30

영문 초록

A new application of high pressure liquid chromatography for the determination of fenitrothion and cis- and trans-neopynamin in insecticidal preparations was investigated. Optimum conditions for a good separation and determination were determined; solvent system: dichloromethane+n-hexane=17+83; Bow rate:0.5ml/min; column: u-porasil (4mm X 30cm) ; absorbance wavelength: 254nm; 0.05 AUFS and sample size:30ul. Recovery of fenitrothion, cis- and trans-neopynamin from mixed artificial preparations was 99.6%, 99.7% and 99.8% respectively. Also reproducibility tests showed that the coefficient of variation was 0.89% for fenitrothion, 0.74% cis-neopynamin and 1.1% for trans-neopynamin. There was no interference with insecticidal preparation containing DDVP, allethrin, S-421 and kerosene. HPLC method was rapid, accurate and it gave better reproducibility and higher sensitivity than any other analytical method. It was considered that HPLC could be greatly applied to the analysis of fenitrothion and neopynamin in insecticidal preparations.