Effects of plant preservative mixture™ on in vitro germination of Dendrobium thyrsiflorum Rchb.f. and its application in orchid conservation

• 영문명: Effects of plant preservative mixture™ on in vitro germination of Dendrobium thyrsiflorum Rchb.f. and its application in orchid conservation
• 발행기관: (사)한국식물생명공학회
• 저자명: Chanh Tran Trung Huy Nguyen Tan Ha Nguyen Thu Le Khanh Hoang Nguyen Huu
• 간행물 정보: 『Journal of Plant Biotechnology』50권 2호, 108~114쪽, 전체 7쪽
• 주제분류: 농수해양 > 기타농수해양
• 파일형태: PDF
• 발행일자: 2023.06.30

영문 초록

In vitro conservation is one of the most effective strategies for rare plant protection, especially for orchid species. To maximize the success rates of in vitro explant establishment (stage I) in conservation programs, the application of tissue culture additives such as Plant Preservative MixtureTM (PPMTM) should be emphasized. In this study, we used Dendrobium thyrsiflorum Rchb.f. (1875) seeds and seedlings as a model for the evaluation of PPMTM’s phyto- toxicity in the meristematic tissues of epiphytic orchids. PPMTM had no observable inhibitory effect on protocorm, shoot, or root development when it was supplemented at 0.1%. PPMTM supplementation caused adverse effects on D. thyrsiflorum explants at concentrations > 0.2%. At high concentrations, young in vitro seedlings showed damage, especially at the root tissue level. Based on this model, supplementation of 0.1-0.2% PPMTM to culture media was successfully implemented to establish in vitro cultures of other rare orchid species in our conservation program.

목차

Introduction

Material and Method

Result and Discussion

Conflict of Interest

Acknowledgement

References

키워드

protocorm micropropagation agriculture production Nervilia