학술논문
아그로박테리움 형질전환법을 이용한 수량증대 유채 식물체 개발
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- 영문명
- Agrobacterium-mediated transformation produces transgenic oilseed rape with a high-yield trait
- 발행기관
- (사)한국식물생명공학회
- 저자명
- 김종보
- 간행물 정보
- 『Journal of Plant Biotechnology』50권 2호, 63~69쪽, 전체 7쪽
- 주제분류
- 농수해양 > 기타농수해양
- 파일형태
- 발행일자
- 2023.06.30
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국문 초록
영문 초록
This study includes the transformation of genes such as ORE7, the increase of gene expression, and the use of the bar gene as a selectable marker that shows herbicide resistance with Agrobacterium tumefaciens using hypocotyls from the oilseed rape “Youngsan” cultivar. To establish an Agrobacterium transformation system for the production of oilseed rape with a high-yield trait, infection time and co-cultivation period with Agrobacterium were tested.
Therefore, when hypocotyls from the oilseed rape “Youngsan” cultivar were infected with Agrobacterium for 20 min and co-cultivated for 3 days, approximately 32-36 putatively transformed hypocotyls with shoots including roots survived from 100 inoculated hypocotyls after 4 weeks of trans- formation on a selection medium containing 20 mg/L of phosphinothricin (PPT) as a selectable agent. Additionally, a PCR assay was performed to confirm the insertion of target genes and showed the presence of the ORE7 gene as a high-yielding trait and the bar gene as a selectable marker.
Treatment with 0.5% (v/v) Basta solution as a selectable agent for 6 days with leaves from transformed oilseed rape expressed the bar gene. Therefore, this study can contribute to the development of special oilseed rapes containing agri- culturally useful traits such as herbicide resistance, drought tolerance, high yielding traits, and high oleic acid content.
목차
서론
재료 및 방법
제초제 저항성(Basta) 검정
통계처리
결과 및 고찰
적요
사사
References
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