아그로박테리움 형질전환법을 이용한 수량증대 유채 식물체 개발

• 영문명: Agrobacterium-mediated transformation produces transgenic oilseed rape with a high-yield trait
• 발행기관: (사)한국식물생명공학회
• 저자명: 김종보
• 간행물 정보: 『Journal of Plant Biotechnology』50권 2호, 63~69쪽, 전체 7쪽
• 주제분류: 농수해양 > 기타농수해양
• 파일형태: PDF
• 발행일자: 2023.06.30

영문 초록

This study includes the transformation of genes such as ORE7, the increase of gene expression, and the use of the bar gene as a selectable marker that shows herbicide resistance with Agrobacterium tumefaciens using hypocotyls from the oilseed rape “Youngsan” cultivar. To establish an Agrobacterium transformation system for the production of oilseed rape with a high-yield trait, infection time and co-cultivation period with Agrobacterium were tested. Therefore, when hypocotyls from the oilseed rape “Youngsan” cultivar were infected with Agrobacterium for 20 min and co-cultivated for 3 days, approximately 32-36 putatively transformed hypocotyls with shoots including roots survived from 100 inoculated hypocotyls after 4 weeks of trans- formation on a selection medium containing 20 mg/L of phosphinothricin (PPT) as a selectable agent. Additionally, a PCR assay was performed to confirm the insertion of target genes and showed the presence of the ORE7 gene as a high-yielding trait and the bar gene as a selectable marker. Treatment with 0.5% (v/v) Basta solution as a selectable agent for 6 days with leaves from transformed oilseed rape expressed the bar gene. Therefore, this study can contribute to the development of special oilseed rapes containing agri- culturally useful traits such as herbicide resistance, drought tolerance, high yielding traits, and high oleic acid content.

목차

서론

재료 및 방법

제초제 저항성(Basta) 검정

통계처리

결과 및 고찰

적요

사사

References

키워드

Agrobacterium high-yield Oilseed rape Selection Transformation