학술논문
고효율의 아그로박테리움 형질전환법을 이용한 제초제저항성 나리 식물체 개발
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- 영문명
- High-efficiency development of herbicide-resistant transgenic lilies via an Agrobacterium-mediated transformation system
- 발행기관
- (사)한국식물생명공학회
- 저자명
- 김종보
- 간행물 정보
- 『Journal of Plant Biotechnology』50권 2호, 56~62쪽, 전체 7쪽
- 주제분류
- 농수해양 > 기타농수해양
- 파일형태
- 발행일자
- 2023.06.30
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국문 초록
영문 초록
Transgenic lilies have been obtained using Agrobacterium tumefaciens (AGL1) with the plant scale explants, followed by DL-phosphinothricin (PPT) selection.
In this study, scales of lily plants cv. “red flame” were transformed with the pCAMBIA3301 vector containing the gus gene as a reporter and the blpR gene as a selectable marker, as well as a gene of interest showing herbicide tolerance, both driven by the CaMV 35S promoter. Using a 20-minute infection time and a 5-day cultivation period, factors that optimized and demonstrated a high transformation efficiency were achieved. With these conditions, approximately 22-27% efficiency was observed for Agrobacterium- mediated transformation in lilies. After transformation with Agrobacterium, scales of lilies were transferred to MS medium without selective agents for 2 weeks. They were then placed on selection MS medium containing 5 mg/L PPT for a month of further selection and then cultured for another 4-8 weeks with a 4-week subculture regime on the same selection medium. PPT-resistant scales with shoots were successfully rooted and regenerated into plantlets after transferring into hormone-free MS medium. Also, most survived putatively transformed plantlets indicated the presence of the blpR gene by PCR analysis and showed a blue color indicating expression of the gus gene. In conclusion, when 100 scales of lily cv. “red flame” are transformed with Agrobacterium, approximately 22-27 transgenic plantlets can be produced following an optimized protocol. Therefore, this protocol can contribute to the lily breeding program in the future.
목차
서론
재료 및 방법
결과 및 고찰
적요
사사
References
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