Analysis of soyasaponin content and biosynthesis-related gene expression in young pea (Pisum sativum L.) sprouts

• 영문명: Analysis of soyasaponin content and biosynthesis-related gene expression in young pea (Pisum sativum L.) sprouts
• 발행기관: (사)한국식물생명공학회
• 저자명: 한경덕 이한결 박재혁 윤영재 김지우 정상윤 문소연 서혜영 김영천 서우덕 이정환
• 간행물 정보: 『Journal of Plant Biotechnology』제50권 제2호, 70~75쪽, 전체 6쪽
• 주제분류: 농수해양 > 기타농수해양
• 파일형태: PDF
• 발행일자: 2023.06.30

영문 초록

In legumes, soyasaponins, one of triterpenoid saponins, are major components of secondary metabolites with a more diverse array of bioactive chemicals. Although the biosynthetic pathway of soyasaponins has been largely studied in soybean, the study on the soyasaponin contents and biosynthesis-related gene expression in pea (Pisum sativum L.) is poorly understood. Here, we found the accumulation of only soyasaponin Bb component in the sprouts of two Korean domestic pea cultivars (Dachung and Sachul). This pattern was consistent with our observation that increased expression of PsUGT73P2 and PsUGT91H4 genes, but not PsCYP72A69, could be responsible for bio- synthesis of only soyasaponin Bb in pea by examining their gene expression. However, gradual accumulation of soy- asaponin Bb at developmental stages was not consistent with the expression of PsUGT73P2 and PsUGT91H4, suggesting that the changes of their protein activities may affect the accumulation patterns of soyasaponin Bb. We also revealed that the increased expression levels of PsUGT73P2 and PsUGT91H4 during light to dark transition led to increase of soyasaponin Bb contents. Collectively, our results provided a molecular basis of metabolic engineering for enhancing useful soyasaponin Bb metabolites in Korean domestic pea cultivars.

목차

Introduction

Materials and Methods

Results and Discussion

Conclusion

Acknowledgements

Conflict of interest

References

키워드

Pea PsCYP72A69 PsUGT73P2 PsUGT91H4 Soyasaponin Bb