Isolation and Characterization of Halophilic Kocuria salsicia Strains from Cheese Brine

• 발행기관: 한국축산식품학회
• 저자명: Hye-Young Youn Kun-Ho Seo
• 간행물 정보: 『Food Science of Animal Resources』Food Science of Animal Resources 제42권 제1호, 252~265쪽, 전체 14쪽
• 주제분류: 농수해양 > 식품과학
• 파일형태: PDF
• 발행일자: 2022.01.31

영문 초록

Kocuria salsicia can survive in extreme environments and cause infections;including catheter-related bacteremia;in humans. Here;we investigated and evaluated the characteristics of nine K. salsicia strains (KS1–KS9) isolated from cheese brine from a farmstead cheese-manufacturing plant in Korea from June to December;2020. Staphylococcus aureus American Type Culture Collection (ATCC) 29213 was used as a positive control in the growth curve analysis and biofilm-formation assays. All K. salsicia isolates showed growth at 15% salt concentration and temperatures of 15℃;25℃;30℃;37℃;and 42℃. KS6 and KS8 showed growth at 5℃;suggesting that they are potential psychrotrophs. In the biofilm-formation analysis via crystal violet staining;KS6 exhibited the highest biofilm-forming ability at various temperatures and media [phosphate buffered saline;nutrient broth (NB);and NB containing 15% sodium chloride]. At 25℃ and 30℃;KS3;KS6;and KS8 showed higher biofilm-forming ability than S. aureus ATCC 29213. The antimicrobial resistance of the isolates was evaluated using the VITEK？？ 2 system; most isolates were resistant to marbofloxacin and nitrofurantoin (both 9/9;100%);followed by enrofloxacin (7/9;77.8%). Five of the nine isolates (5/9;55.6%) showed multidrug resistance. Our study reports the abilities of K. salsicia to grow in the presence of high salt concentrations and at relatively low temperatures;along with its multidrug resistance and tendency to form biofilms.

목차

Introduction

Materials and Methods

Results and Discussion

Conclusion

Conflicts of Interest

Acknowledgments

Author Contributions

Data Availability

Ethics Approval

References

키워드

Kocuria salsicia cheese brine growth curve biofilm antimicrobial resistance