Regulation of Metalloprotease VvpE Production by Quorum-sensing System in Vibrio vulnificus

• 발행기관: 조선대학교 의학연구원
• 저자명: Choon-Mee Kim Joon-Seob Song Sung-Heui Shin
• 간행물 정보: 『The Medical Journal of Chosun University』제33권 제2호, 48~55쪽, 전체 8쪽
• 주제분류: 의약학 > 기타의약학
• 파일형태: PDF
• 발행일자: 2008.06.30

영문 초록

Expression of vvpE encoding a metalloprotease called VvpE is known to be positively regulated by the autoinducer-2-mediated quorum-sensing system (AI2-QSS). Recently, we found that both 35 and 45 kDa-VvpE, the authentic products of wpE, are produced within bacterial cells and thereafter secreted, and moreover, 35 kDa-VvpE is the major secreted form. In this study, we attempted to observe the effect of AI2-QSS on the production profile of VvpE. VvpE production was decreased along with the down-regulation of vvpE transcription by a mutation of luxS encoding an enzyme (LuxS) required for the synthesis of AI2 or of smcR encoding a master transcription activator (SmcR) of AI2-QSS. The decreased VvpE production was rescued by in trans complementation of wild-type luxS or smcR. However, the production profile of VvpE was not affected by mutation of luxS or smcR. These results indicate that AI2-QSS regulates VvpE production by controlling vvpE expression, but does not affect the production profile of VvpE.

목차

INTRODUCTION

MATERIALS AND METHODS

RESULTS AND DISCUSSION

키워드

Vibrio vulnificus Metalloprotease Quorum-sensing system Autoinducer-2