사람의 비후성 반흔에서 면역조직화학기법에 의한 형질전환인자 알파와 표피성장인자 발현의 차이에 관한 면역조직화학적 연구

• 영문명: Differences of Expression between Transforming-growth factor-a and Epidermal growth factor in Human Hypertrophic Scan An Immunohistochemical Study
• 발행기관: 조선대학교 의학연구원
• 저자명: 박영란(Young-Lan Park) 김현곤(Hyun-Kon Kim) 김종중(Jong-Joong Kim) 문정석(Jeong-Seok Moon) 송준섭(Joon-Seob Song) 설
• 간행물 정보: 『The Medical Journal of Chosun University』제33권 제2호, 29~36쪽, 전체 8쪽
• 주제분류: 의약학 > 기타의약학
• 파일형태: PDF
• 발행일자: 2008.06.30

영문 초록

Background and Objectives: Transforming growth factor-a (TGF-a) and epidermal growth factor (EGF) are polypeptides which interact with the epidermal growth factor receptor (EGFR) to produce their biological effects. The aim of the present investigation was to elucidate the immunolocalization of TGF-a and EGF in normal human skin, hypertrophic scar with skin graft on soft tissue defect, and postbum hypertrophic scar without graft. Methods: The data presented in this paper focused attention on differences of expression between two kinds of hypertrophic scars in relation to skin graft using immunohistochemistry. Formalin-fixed and paraffin-embedded tissues from 3 normal skin tissues, 3 hypertrophic scars after skin graft and 8 postbum hypertrophic scars without graft were immunolabelled with antibodies directed against TGF-a and EGF. Results: In normal epidermis of skin, strong TGF-a immunoreactivity (IR) was observed in all epidermal layers except the stratum (S.) basale, whereas EGF was immunopositive in a few cells over all epidermal layers. The staining for TGF-a was found in cell membrane, and EGF was seen either diffuse cytoplasmic or peripheral part of cells in normal epidermis. In hypertrophic scar after six months postoperation, TGF-a IR was observed in the upper part of S. spinosum and S. comeum，whereas EGF was diffusely expressed in the S. spinosum, also its intensity was slightly increased compared to normal skin. In hypertrophic scar without skin graft, very strong expression of EGF was detected in the S. spinosum and S. comeum, and intensity of EGF was increased when compared to normal skin and postoperative hypertrophic scar. EGF and TGF-a coexisted in the cells of S. spinosum in normal skin as well as hypertrophic scars. Double-labeled cells were increased in postbum hypertrophic scar compared to normal skin and postoperative hypertrophic scar. In addition, intensity of EGF and TGF-a double IR was the strongest in postbum hypertrophic scar. Conclusion: These results indicate that EGF may have a more complex regulatory role not only in the early stages of wound healing but also in hypertrophic scar.

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키워드

Transforming growth factor-a Epidermal growth factor Skin Hypertrophic scar Immunohistochemistry