유전자 재조합 대장균을 이용한 페닐알라닌 생산성 및 DAHP 합성효소의 활성도 검토

• 영문명: A Study on the Phenylalanine Productivity and DAHP Synthase Activity by Recombinant Gene in Escherichia coli
• 발행기관: 한국산업기술융합학회(구. 산업기술교육훈련학회)
• 저자명: 고하영(Ha Young Koh)
• 간행물 정보: 『산업기술연구논문지』산업기술교육훈련논문지 제18권 4호, 19~25쪽, 전체 7쪽
• 주제분류: 공학 > 공학일반
• 파일형태: PDF
• 발행일자: 2013.12.30

영문 초록

The production of aromatic amino acids were regulated by 3 initial regulatory enzymes thoses were 3-deoxy-D-arabinoheptulose-7-phosphate synthetases which codes by Phenylalanine, Tyrosine, Tryptophan and named aroG, aroF, aroH respectively. The plasmid, pSY160-14 including the aroG gene has newly constructed and is transferred to AT2471. DAHP synthetase activity with aroG gene in host cell was detected 22% higher compared to pSY130-14 at 37℃. As these results, it appears that the plasmid with aroG gene was considered directly regulated and inhibited to DAHP synthetase and also observed the Phenylalanine productivity and gene expression were detected 21%, 22% respectively better than control below 40℃.

목차

ABSTRACT

Ⅰ. 서론

Ⅱ. 실험재료 및 방법

Ⅲ. 실험결과 및 고찰

Ⅳ. 결론

참고문헌

키워드

플리스미드 DAHP 합성효소 아로지 유전자 페닐알라닌 Plasmid DAHP Synthase aroG gene Phenylalanine